Day's Lock is a lock on the River Thames near Dorchester-on-Thames, Oxfordshire, England on the Dorchester side of the river.

The lock is across the river from the small village of Little Wittenham and is overlooked from the south by the hills of Wittenham Clumps, with a particularly good view from Round Hill.

A short way downstream of the lock is Little Wittenham Bridge which spans the river in two sections with an island between. Using this, the lock is a short walk from Little Wittenham.

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